MultiWash+ Microplate Washer. 2. UV absorbance detection. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. The FilterMax F3 can read 96-well and 384-well plates. Efficiency. We would like to show you a description here but the site won’t allow us. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. It. 00. 96 deep well plate: packed in sleeves of 6 , 24 per case. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes <2 fg/well for firefly luciferase in 96- and 384- well top read. Can read 96 well plates (optional 384 well). , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today. 384-Deep Square Well Plates. 057, depending on lot number. All stand-alone instrument functions can be controlled using the software. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. 1. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Microplate readers are commonly used in life science laboratories. Microplates for applications that require high sample recovery rates. 40266. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Expanded wavelength coverageIn the software select Tools > Configuration. x. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. 0200-6263. Values are averages from 5 clean plates, each filled with 200 µL water/well. (blue) and DsRed (red) transfected cells in 384-well plates, read from the bottom. ,LTD (CAT#: STEM-S. EMax. A. Full-skirted standard or extra. Overview of 384 Well SBS. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Fluorescence Microplate Reader Comparison Chart. ability to use as little as 2˜µL samples with 24- or 64-well plates. C. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. #ABS Plus). B. Read one sample or up to 384. This enhanced capability. 384 well plate (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Devices PathCheck® Sensor—the only patented technologySpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices). SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). Overview. Supplier: Molecular Devices. " A versatile UV/VIS spectrophotometer for both 96- and 384-well. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Plate Formats: 6- to 384-well plates. 96-, 384-, and 1536. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. x. Two different agonists, acetylcholine and carbachol, were tested. Alpha 384 HTS 96- and 384-well . EPOCH2 Plate Reader/Spectrophotometer. Packed 25 plates per sleeve, 2 sleeves per case. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Polypropylene deep well plates: Elevated well walls for easier sealing Round. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. 96-, 384-, and 1536-well microplates. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 984. Total volume (except deep. ©2022. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Molecular Devices has provided scientists with tools to expand the boundaries of their research. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. Temperature control: 4°C above ambient to 45°C. Access this by going to. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. Total volume (except deep well plates) is 120 mL. CO 2 & O 2 control. Efficient washing is achieved with cross-wise aspiration, reducing residual volume . Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. 2. There is no product in the shopping cart, buy it!. Compare specifications and request a quote from leading suppliers. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Description. We would like to show you a description here but the site won’t allow us. Made from polypropylene with conical well profiles to maximize recovery and allow working with sample volumes as low as 5 µL. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. A rinse program is included for quick and easy daily. Plate chamber design of the SpectraMax category 1 microplate readers. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)…Elisa Plate Readers and Microplate Readers at Biocompare. 6-well tissue-culture treated (TC) plate, sterile. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 20 µL (96-well plates) or 10 µL (384-well plates. x. 3. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Versa Max. Pre-read measurements were made with 200 µL water in each well of a Costar UV plate, after which the plate was emptied and blotted dry. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. 6% for the on-board optics (monochromators). Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Visit the supplier page or submit a quote to learn more. After reading the ELISA plate on both readers, each standard curve was nearly identical. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Outer wells aligned for proper viewing with FLIPR Ideal. The system provides automation for up to 50 microplates for easy walkaway automation. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. the use of a reader calibrated with primary NIST and NMI standards. (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). 190. For more sample throughput, both readers can be easily. Plus 384. 384-Well Microarray/High Sample Recovery Plate. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. This unlocks the transport slide. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Detection cartridge modularity allows for easy addition of new read modes within minutes without. In addition, the iD5 reader measures TRF and FP and. 20 attomol ATP per well. Dimensions: 18. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Round well design with a clear flat bottom 2. 48 ± 0. Microplates for applications that require high sample recovery rates. T = I/I 0 and %T = 100 (T). 312 gain calibration failed. Also refer to this Multimode Reader Comparison. Synergy NEO multi-mode plate reader: BioTek: n/a: EL406 plate washer and bulk dispenser with wide-bore tip 10 μL cassette: BioTek: n/a:. 384-Well Microarray/High Sample Recovery Plate. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. There is no product in the shopping cart, buy it!. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. 6-well tissue-culture treated (TC) plate, sterile. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 1: The SPECTRAmax 340PC 384 System The on-board microprocessor calculates and reports the absorbance (or % Transmittance) for each well of a microplate. 305 reference level saturation. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. They perform rapid throughput cell-based assays while. Plate reader instructions Computer. Select Update Driver. 5–25 µl. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . Others models hold the sample in place using surface tension. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. Both readers were heated to 37ºC prior to setting up the assay. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Molecular Devices has provided scientists with tools to expand the boundaries of their research. 2. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andThe Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. (or plate reader). This reader also makes use of a unique temperature. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. To update the driver. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Molecular Devices has developed the FLIPR Membrane Potential Assay Kits to maximize. Method 1. 5 pM 2. x. Molecular Devices products are warranted to meet the stated specifications. The FLEXstation II 384 (Molecular Devices, Inc. Plate formats. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. Figure 1. A 96- and 384-well automated washer. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. Tunable wavelength selection / monochromator. These advertising cookies will no longer be set for this browser and device. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. -Flex3 link -we don't have this) complements the Tecans, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. Option 1: Use a plate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. An industry-renowned instrument for monitoring GPCRs and ion channels. Get Quote . The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 039. The MultiWash+ Microplate Washer is an automated washer that is configurable for both 96- and 384- well plates. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 2, prime the machine with CellTiter-Glo ® 2. Select Yes at the warning. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. x. 340 PC384. This eliminates the need for standard curves, and Flexibility to read a variety of plate formats. Rating: 3. Dual injectors for kinetic assays. 1 fmol luciferase per well. 19 H mm that meet microplate industry standards. The FLIPR 384 system comes complete with 384. Microplate readers can be used to quantify this turbidity to monitor events such as cell growth or to perform solubility screens. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. 6-384 (96- and 384-well with pipetting) 96-well microplates. These lab workhorses are used in drug discovery, research, bioassay validation, and. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. 8 mm distance between well bottoms to plate bottom. M2. 2 with 1000 cells. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. By measuring optical pathlength, it. These robust readers have been placed in labs from Antarctica to the. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. for the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. 3. SpectraMax® Plus 384 provides ultrafast, full spectral range. 25mm: Plate height:Absorbance was measured at 490 nm with SpectraMax Plus 384 plate reader (Molecular Devices, San Jose, CA). They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 384 Well Plates; Wavelength(s): 200 to 1000 nm (Abs), 250 - 850 nm (Fluor) Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. 0 . Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Industry standard disposable (choose from recommended list). Or scan up to 384 wells at any time to determine the best assay conditions. x. 12, 24, 96 & 384-well SBS microplates; Range of 96, 384-well SBS microplates . More Product Information. x. Devices’ StakMax® Microplate Stacker in a matter of minutes and . It also integrates with the. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Temperature control. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. The default pipettor dispensing speed is 50 µL/sec for 96-well plates and 20 µL/ sec for 384-well plates. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. 1 a. Add 10 µL of ADP Detection Mix to those rows. and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wave-length range (spectral scan). 1 x plates. FlexIII. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 96-well fluorescent cell dilution series, top-read. VMax . SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. DynaPro ™ Plate Reader III. Category 2 and 3 microplate readers use gradient heating as well. X. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. x. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Important things to consider when purchasing a multimode microplate reader. 003 fmol 0. UV absorbance detection. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. x. For nearly 30 years, Molecular Devices has provided scientists with . Cross-talk <0. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. USA. 96- and 384-well . Dilution series for ZsGreen (green), AcGFP (blue) and DsRed (red) transfected cells. For more sample throughput, the system can384 Well Plate, supplied by Molecular Devices LLC, used in various techniques. 96-1536. 2. The plate reader consisted of a large-area. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The price of a microplate reader depends on the technical equipment and the number of measurement technologies that the device can measure. VMax . 2. Related products. All stand-alone instrument functions can be controlled using the software. SpectraMax i3x. In simplest terms, a plate reader measures and. For biochemical assays, the washer removes unbound material and unreacted reagents. Molecular Devices: n/a:. 6-384 (96- and 384-well with pipetting) 96-well microplates. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. MultiWash+ Microplate Washer. 384-well TC plate, Poly-d-Lysine, Flat. With optimized reagents, option to operate the. 96-384. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Washers configured for both 96- and 384-well plates. 0 OD for earlier models. 340 PC384. 11 10 100 1000 0 20 40 60 80 100 120 Calcium Mobilization in WT3M1 CHO Cells EC 50 = 13. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. Figure 2-23: Bottle holder To install the bottle holder: 1. Spectranet customer portalThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. All microplate washers enable users to program tank selection, volumes, step times, and cycles for customized procedures. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Absorbance cuvette port. That explains the lower values on the LmaxII384 plate reader. Service Contracts, Preventative Maintenance and. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. All stand-alone instrument functions can be controlled using the software. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. Introduction. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. #i3X) with: SoftMax Pro 7. Compact design. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Turn the. e. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. System setup and calibration are controlled from within SoftMax Pro Software. Turn the reader on and secure the USB cable. x. flat meniscus), but is approx 15 mL. 1 OD at 230 nm. The third tested COC-based plate, the UltraVision 384-well microplate, however, absorbed at 2 OD at 200 nm,. These models can no longer be repaired. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Browse for different fluorescent readers below and compare across different instrument suppliers. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. For more sample throughput, the system canPlate Background OD, which was used for subsequent tests, was measured as follows. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in HTS and lead optimization applications. ZERO BIAS - scores, article reviews, protocol conditions and more. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Ensure your assay quality with quality tips Certified RNase-/DNase-free Nonpyrogenic Nonsterile 50 racks/case. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. 384-WELL 10MM-HEIGHT HIGH SAMPLE RECOVERY MICROPLATES WI/WO LID. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. 616, 665 nm EM filters 0200-7011. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Dual injectors for kinetic assays. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. FlexIII. This can increase throughput and efficiency, thus saving time and space in the lab. 0 and dispense 10 μL to all wells of the 384-well plate(s) (see Note 13). The. Selects either 96-well or 384-well plate for a stand-alone read. X. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices'. The modular platform allows upgrading to expand functionality as your laboratory workflows change. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Published Dec 16, 2020 | Updated Dec 16, 2020. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Using Molecular Devices microplate readers and SoftMax. 6-384. Temperature Control: ambient from +4 to 45C. Includes PC and software. Products : Ionworks HT, Ionworks Quattro. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. x. 48 W x 12. Temperature control: 4°C above ambient to 45°C. It features a modular design that allows users to add or change detection cartridges according to their needs. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. 3860 N First Street.